AB SCIEX TripleTOF 5600 is a powerful instrument that provides high mass accuracy and high resolution in both MS and MS/MS modes. With the ability to acquire a maximum of 50 MS/MS spectra per second, the instrument makes a great component for a proteomics research platform.
PEAKS algorithm has been optimized specifically for this type of instrument in version 6. A comparative study was performed using iPRG 2012 dataset against Mascot and ProteinPilot.
At 1% FDR, PEAKS 6 was able to identify twice as many PSMs as the popularly used Mascot + Percolator combination. Even compared with AB SCIEX’s ProteinPilot software, PEAKS 6 identified 29% more PSMs.
*The content of this post is extracted from ASMS 2012 poster "Optimized Database Search Software for Peptide Identification with AB SCIEX TripleTOF 5600". You can find a copy of the poster here.
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That's an impressive endorsement of the AB SCIEX TripleTOF 5600 and PEAKS 6 algorithm! The performance boost, particularly in terms of peptide spectrum matches (PSMs), is remarkable. This combination makes it an excellent choice for proteomics research. If you ever need assistance in exploring this powerful instrument or related topics, our Assignment Helper team is here to provide expert guidance and support.
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