Thursday, July 4, 2013

How to use Decoy-Fusion on Mascot in PEAKS inChorus?

PEAKS supports FDR estimation on inChorus results. To make this work on Mascot results, there are a few extra steps to follow.

PEAKS uses Decoy-Fusion method for FDR estimation. The first step is to create a decoy-fusion database. Go to PEAKS database configuration dialog. Select the FASTA database you want to search against. Then click the "Export Decoy DB" button. A decoy-fusion FASTA file will be generated.

The second step is to configure the decoy-fusion FASTA file into Mascot. This is very straightforward in Mascot 2.4 as the parsing rule of PEAKS decoy-fusion method can be automatically detected.

After the decoy-fusion database is up and running on Mascot server, the last step is to make sure that the "Search decoy database from PEAKS" option is selected in the search dialog.


  1. I do elieve all the concepts you’ve introduced on yohr post. They’re really convincing and can definitely work.

    Nonetheless, the posts are too quicck for newbies. May you please extend themm a bit from next time? Thank you for the post.

  2. hey! this is andy,and i am here to help the students as i am also a student i thought that every student is facing a lot of problem in their assessments and for them i am here mentioning professional essay writers service by contacting them you will get the best help of which you always desired for.