Some researchers are looking for unknown amino acids in their proteomic studies. This requires the software to be able to take new theoretical AA into account. Although, PEAKS internally only use the 20 common amino acids residues, here is a trick we can use to add new AAs.
The general concept is to add this new amino acid X as a PTM of an existing amino acid Y. The existing amino acid Y should be picked so that the mass difference between X and Y is distinguishable from the mass of all PTMs in the search. In the analysis, on top of the normal PTMs you may set, set this special PTM as a variable modification. In the result, this new amino acid X will appear as a variable modification of Y.
Here is an example. Let's add the amino acid Pyrrolysine to PEAKS. Open the PTM configuration by click the menu "Window"-->"Configuration", then select the PTM tab. Click the "New" button on the lower right corner of the dialog. I am going to configure it as a modification of Alanine. The mass difference is 184.1212Da.
Click "OK" to complete the configuration. When performing a PEAKS search, the new PTM, Pyrrolysine, can then be selected in the "Customized" tab of the "PTM Options" dialog.
Use the same trick, we can substitute or remove an amino acid by setting it as a fixed modification in the search.
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With PEAKS, you may unlock the potential of your study by adding new and non-standard amino acids. Improve your analyses, venture into unexplored territory, and stay on the cutting edge of amino acid research. With PEAKS, you can embrace the future of discovery.
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